Toronto, ON
40d ago



Reference : R2453977

Location : Sanofi Pasteur Limited / North York, Ontario

Address : 1755 Steeles Ave W, North York, ON M2R 3T4

Department : IPV

Reporting To : IPV Production Manager

Required Education : University Degree (Microbiology, Biochemistry, Virology, Immunology, Engineering)

Sanofi Pasteur, the vaccines division of Sanofi, is the largest company in the world devoted entirely to human vaccines.

For over 100 years, we’ve provided life-saving vaccines for the prevention and treatment of infectious diseases.

Sanofi Pasteur Limited, located in Toronto, is one of the company's four major R&D and manufacturing sites. We are home to Sanofi Pasteur's North American centre for Analytical and Bioprocess R&D.

As well, we are the largest Canadian vaccine producer : each year, we manufacture 50 million doses of vaccine and distribute them to more than 90 countries around the world, helping to protect against 20 infectious diseases.

This variety of activities offers employees diverse opportunities for personal and professional growth. Our company attracts some of the best minds in the business from across the globe.

Our 1,300 employees give us their best and we offer the best in return.

Sanofi Pasteur Limited is looking for motivated and results driven individuals to join our IPV Department as a Technologist.

Key responsibilities will include the following :

Position Summary :

1. Operation of Fermentation Equipment

  • Cleaning, setup and maintenance of 3x 1000L fermenters; must be willing to work in a confined space.
  • Perform sterilization and decontamination of pressure vessels. Must be able to operate process control equipment and understand principles of sterilization.
  • Aseptic addition of media and micro carrier beads to fermenters.
  • Maintenance of ancillary fermentation (e.g. Pumps, filter housings, tubing).
  • 2. Mammalian Cell Culture and Virus Propagation

  • Monitoring, documenting, and adjustment of fermentation parameters, verification of setpoints may require reprogramming of EFC 24 control system.
  • Aseptic sampling of fermenters, observation of tissue culture microscopically and assessment of health of cells, cell counting, determination of CPE.
  • Calculation of seed virus required for inoculation (requires understanding of logs / antilogs).
  • Filtration of media and aseptic addition of cell suspension to fermenters, seed virus addition, media addition.
  • 3. Downstream Processing of IPV : Filtration, Concentration and Chromatographic Purification.

  • Perform clarification and filtration of virus harvests.
  • Perform concentration of virus harvests using ultrafiltration apparatus.
  • Decontaminate liquid waste stream prior to discharge.
  • Perform gel filtration and ION exchange chromatography purification steps.
  • Perform concentration and clarification of purified virus.
  • Prepare and maintain large capacity pumps, filter housings, chromatography columns.
  • Must have knowledge of column packing and be able to perform HETP calculations.
  • Clean, assemble and sanitize UF equipment and prepare sanitizing solutions.
  • 4. Inactivation.

  • Calculation of media volume required for concentrate dilution.
  • Dilution, Filtration and Warming of the purified Virus Pool.
  • Calculations for volume of Formaldehyde required and formaldehyde dilutions.
  • Addition of Formaldehyde.
  • Aseptic sampling of Product during inactivation.
  • Filtration, Completion of Inactivation and Storage of Injectable Polio.
  • Transport of Inactivated Polio Monovalents.
  • Dialysis of samples for Cell culture safety testing.
  • 5. Filing and Documentation.

  • Document and file all necessary paper work required for BPR preparation, maintenance logs, cleaning logs, etc. Ensure information is up-to-date and accurate.
  • Review and requisition as necessary through Physical Plant, Materials Management, Stores, Media Department- all necessary reagents and equipment.
  • Perform all required MRP and LINQ / LIMS duties.
  • 6. General Duties

  • Operation of the Decontamination autoclave, magnehelic gauges.
  • Environmental Monitoring, WFI sampling, Sample delivery to QC laboratories.
  • Familiarity and compliance with Gowning procedures and material / equipment / personnel flows.
  • Education and Experience :

  • Minimum four (4) year University Degree (Microbiology, Biochemistry, Virology, Immunology, Engineering)
  • Minimum of one (1) year of previous relevant experience in a Biotechnology, Pharmaceutical company, or University Research Institution.
  • Demonstrated ability to work independently and make quick decisions in accordance with SOPs and training.
  • Basic computer skills and the ability to enter data accurately and in a timely manner.
  • Must be proficient in Microsoft Excel
  • Hours of Work :

    Monday to Friday 7 : 00a.m.-3 : 00p.m.

    Sanofi is an equal opportunity employer committed to diversity and inclusion. Our goal is to attract, develop and retain highly talented employees from diverse backgrounds, allowing us to benefit from a wide variety of experiences and perspectives.

    We welcome and encourage applications from all qualified applicants. Accommodations for persons with disabilities required during the recruitment process are available upon request.

    Thank you in advance for your interest.

    Only those candidates selected for interviews will be contacted.


    Sanofi is dedicated to supporting people through their health challenges. We are a global biopharmaceutical company focused on human health.

    We prevent illness with vaccines, provide innovative treatments to fight pain and ease suffering. We stand by the few who suffer from rare diseases and the millions with long-

    term chronic conditions.

    With more than 100,000 people in 100 countries, Sanofi is transforming scientific innovation into healthcare solutions around the globe.

    Sanofi, Empowering Life

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